Table 1. Regeneration of eleven Cyclamen-genotypes using protocol 1. |
Genotype / Source of explants |
Number of explants |
Number of sterile explants |
Start of differentiation after months |
Explants with somatic embryo-like structures or shoots* |
Number of plantlets after 7 months |
|
|
|
|
|
% |
total |
|
C. africanum |
|
|
|
|
|
|
Placenta |
8 |
8 |
0 |
0 |
|
0 |
Peduncle |
8 |
4 |
3 |
50.0 |
2 |
0 |
Leaf |
8 |
7 |
0 |
0 |
|
0 |
Petiole |
20 |
16 |
7 |
12.5* |
2 |
2* |
C. cilicium |
|
|
|
|
|
|
Leaf |
8 |
4 |
0 |
0 |
0 |
|
Petiole |
20 |
20 |
6 |
15.0 |
3 |
1 |
C. coum |
|
|
|
|
|
|
Placenta |
36 |
24 |
3 |
4.2 |
1 |
8 |
Peduncle |
36 |
20 |
0 |
0 |
|
0 |
Leaf |
8 |
6 |
0 |
0 |
|
0 |
Petiole |
28 |
19 |
6 |
15.8 |
3 |
3 |
C. coum ssp. coum |
|
|
|
|
|
|
Leaf |
20 |
7 |
0 |
0 |
0 |
|
Petiole |
24 |
20 |
6 |
5.0 |
1 |
3 |
C. coum magenta |
|
|
|
|
|
|
Placenta |
20 |
20 |
3 |
30.0 |
6 |
249 |
Peduncle |
20 |
12 |
3 |
25.0 |
2 |
200 |
Leaf |
20 |
10 |
6 |
20.0 |
2 |
10 |
Petiole |
24 |
20 |
3 |
10.0 |
2 |
3 |
C. hederifolium ssp confusum |
|
|
|
|
|
|
Placenta |
4 |
4 |
0 |
0 |
0 |
|
Peduncle |
4 |
4 |
0 |
0 |
0 |
|
Leaf |
4 |
4 |
6 |
25.0 |
1 |
1 |
Petiole |
16 |
14 |
6 |
14.3 |
2 |
8 |
C. hederifolium cv. ‘Perlenteppich’ |
|
|
|
|
|
|
Placenta |
20 |
11 |
0 |
0 |
|
0 |
Peduncle |
20 |
8 |
0 |
0 |
|
0 |
Leaf |
16 |
7 |
0 |
0 |
|
0 |
Petiole |
20 |
16 |
3 |
12.5 |
2 |
2 |
C. hederifolium cv. ‘Rosenteppich’ |
|
|
|
|
|
|
Placenta |
20 |
10 |
0 |
0 |
0 |
|
Peduncle |
20 |
7 |
7 |
42.9 |
3 |
3 |
Leaf |
16 |
12 |
0 |
0 |
|
0 |
Petiole |
20 |
20 |
0 |
0 |
|
0 |
C. persicum |
|
|
|
|
|
|
Placenta |
20 |
16 |
3 |
12.5 |
2 |
10 |
Peduncle |
20 |
4 |
3 |
50.0 |
2 |
8 |
Leaf |
4 |
4 |
0 |
0 |
|
0 |
Petiole |
16 |
16 |
0 |
0 |
|
0 |
C. persicum pink |
|
|
|
|
|
|
Leaf |
4 |
4 |
0 |
0 |
|
0 |
Petiole |
16 |
15 |
0 |
0 |
|
0 |
C. purpurascens |
|
|
|
|
|
|
Placenta |
20 |
15 |
3 |
6.7 |
1 |
16 |
Peduncle |
20 |
20 |
3* |
20.0* |
4 |
13* |
Leaf |
12 |
12 |
6* |
25.0* |
3 |
50* |
Petiole |
20 |
20 |
3* |
25.0* |
4 |
71* |
* Regenerants were classified as shoot formation |
|