Plant Biotechnology
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 13 No. 1, Issue of January 15, 2010
© 2010 by Pontificia Universidad Católica de Valparaíso -- Chile Received January 12, 2009 / Accepted September 8, 2009
DOI: 10.2225/vol13-issue1-fulltext-1
RESEARCH ARTICLE

In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC. 

José Luis Piña-Escutia
 Centro de Investigación y Estudios Avanzados en Fitomejoramiento
Facultad de Ciencias Agrícolas
Universidad Autónoma del Estado de México
Toluca, Estado de México, México

Luis Miguel Vázquez-García
 Centro de Investigación y Estudios Avanzados en Fitomejoramiento
Facultad de Ciencias Agrícolas
Universidad Autónoma del Estado de México
Toluca, Estado de México, México

Amaury Martín Arzate-Fernández*
 Centro de Investigación y Estudios Avanzados en Fitomejoramiento
Facultad de Ciencias Agrícolas
Universidad Autónoma del Estado de México
Carretera Toluca-Ixtlahuaca km 11.5
Campus Universitario “El Cerrillo” 50200
Toluca, Estado de México, México
E-mail: amaury1963@yahoo.com.mx

*Corresponding author

Financial support: We are grateful to Consejo Nacional de Ciencia y Tecnología (CONACYT) from México for the scholarship give to JLPE (2005-183935), and to the RED TIGRIDIA (SINAREFI) for covering the publishing payment.

Keywords: genetic integrity, micropropagation, oceloxóchitl.

Abbreviations:

2,4-D: 2,4-Dichlorophenoxiacetic acid
AC: activated charcoal
BAP: 6-Benzylaminopurine
ISSR: Inter Simple Sequence Repeats
MS: murashige and skoog
NAA: Naphtalen Acetic Acid
N6: Chu basal medium
PCR: Polymerase Chain Reaction
Abstract   Full Text

Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.
Supported by UNESCO / MIRCEN network