In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.
José Luis Piña-Escutia
Luis Miguel Vázquez-García
Financial support: We are grateful to Consejo Nacional de Ciencia y Tecnología (CONACYT) from México for the scholarship give to JLPE (2005-183935), and to the RED TIGRIDIA (SINAREFI) for covering the publishing payment.
Keywords: genetic integrity, micropropagation, oceloxóchitl.
Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.