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Genetic diversity of breeding popcorn lines determined by SSR markers Ana Paula Ribeiro
Trindade Ronald José Barth Pinto Antonio Teixeira do
Amaral Júnior Claudete Aparecida Mangolin Maria de Fátima Pires da Silva Machado Carlos Alberto Scapim* *Corresponding author Financial support: National Counsel of Technological and Scientific Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES), and Fundação Araucária - Foundation in Support of the Scientific and Technological Development of the State of Paraná, Brazil. Keywords: advanced generations, genetic variability, molecular markers, Zea mays.
Information about genetic dissimilarity is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations. Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers. Of a total of 51 evaluated polymorphic primers, 15 were used for polymerase chain reaction (PCR) amplification. The genetic distance was estimated by Rogers’ modified distance. The different popcorn breeding programs in Brazil are possibly using highly similar base-populations. The genetic similarity of lines P1-3 and P8-1 was lowest, while P3-3 and P8-2 were genetically more similar. The cophenetic correlation showed that the Unweighted Pair-Group Method Using Arithmetic Averages (UPGMA) was reliable to discriminate the genotypes in five groups. The clusters were consistent with the estimates of genetic identity. There was a moderate coincidence degree between the groups and genealogy of lines. Higher levels of heterozygosity are expected from crosses between the group containing lines P3-3 and P7-3 with that of P1-3 and P7-4. Crosses between lines P1-3 and P8-1 are also promising. |