Plant Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 13 No. 1, Issue of January 15, 2010
© 2010 by Pontificia Universidad Católica de Valparaíso -- Chile Received December 22, 2008 / Accepted July 12, 2009
DOI: 10.2225/vol13-issue1-fulltext-4
REVIEW ARTICLE

Induction of somatic embryogenesis as an example of stress-related plant reactions

Maria Amely Zavattieri*
Laboratory of Plant Breeding and Biotechnology
Institute of Mediterranean Agricultural and Environmental Sciences
University of Évora
Évora, Portugal
E-mail: zavattieri@uevora.pt

António Miguel Frederico
EU Marie Curie Chair
Institute of Mediterranean Agricultural and Environmental Sciences
University of Évora
Évora, Portugal

Mónica Lima
Laboratory of Plant Breeding and Biotechnology
Institute of Mediterranean Agricultural and Environmental Sciences
University of Évora
Évora, Portugal

Rui Sabino
Institute of Mediterranean Agricultural and Environmental Sciences
University of Évora
Évora, Portugal

Birgit Arnholdt-Schmitt
EU Marie Curie Chair
Institute of Mediterranean Agricultural and Environmental Sciences
University of Évora
Évora, Portugal

Website: http://www.icam.uevora.pt

*Corresponding author

Financial support: Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM),University of Évora, 7002-554 Évora, Portugal.

Keywords: cell reprogramming, reactive oxygen species, somatic embryogenesis, stress.

Abbreviations:

2,4-D: 2,4-dichlorophenoxyacetic acid
ABA: abscisic acid
ABI3: ABI gene family member 3
ANP1: Arabidopsis NPK1-like protein kinase
AP: ascorbate peroxidase
AOX: alternative oxidase
DNA: deoxyribonucleic acid
Fe-EDTA: ferric ethylenediaminetetraacetic acid
M: molar concentration
MAPK: mitogen-activated protein kinase
NO: Nitric oxide
PGRs: plant growth regulators
ROS: reactive oxygen species
SE: somatic embryogenesis
SOD: superoxide dismutase

Abstract   Full Text

In this review, we address the role of stress as one of the principal causes for a cell or tissue to change its pre-existing somatic program, reprogramming itself to express the embryogenic pathway. The focus of this paper is the effect of different stress conditions on the induction phase of plant somatic embryogenesis, as well as the development of embryogenic competence as a result of the applied stresses. We also present a variety of data that link plant somatic embryogenesis, DNA methylation and oxidative stress response.

Supported by UNESCO / MIRCEN network