Microbial Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 13 No. 1, Issue of January 15, 2010
© 2010 by Pontificia Universidad Católica de Valparaíso -- Chile Received february 2, 2009 / Accepted August 23, 2009
DOI: 10.2225/vol13-issue1-fulltext-5

Sequential use of ammonium and leucine as nitrogen sources during growth of Geotrichum candidum on a glucose based medium

Lydia Adour
Laboratoire de Chimie Appliquée et de Génie chimique
Université Mouloud Mammeri
Tizi-Ouzou, Algeria

Florence Bude
Département Chimie, IUT de Rennes
Université de Rennes
Rennes, France

Abdeltif Amrane*
Ecole Nationale Supérieure de Chimie de Rennes
Université de Rennes
CNRS, UMR 6226,
Avenue du Général Leclerc
CS 50837, 35708 Rennes Cedex 7, France
E-mail: abdeltif.amrane@univ-rennes1.fr

Website: http://www.ensc-rennes.fr/recherche/cip/

*Corresponding author

Keywords: amino acids, batch culture, diauxic growth, Geotrichum candidum, growth kinetics.

Abstract   Full Text

Geotrichum candidum growth on ammonium and leucine as nitrogen sources and glucose as a carbon source was examined. A clear preference of G. candidum for ammonium over leucine as a nitrogen source was shown. Indeed, ammonium was completely exhausted at the end of exponential growth after less than 35 hrs of culture; in contrast only 5% of leucine was concomitantly assimilated. Growth continued at slower rates on glucose and leucine as carbon and nitrogen sources respectively, and at the end of culture (185 hrs), leucine was completely exhausted.

Supported by UNESCO / MIRCEN network