Enhancement of wanlongmycin production by nitrogen ion beam implantation

Xiaoyong Zhang
Plant Protection Research Institute
Guangdong Academy of Agricultural Science and
Chengdu Institute of Biology
Chinese Academy of Sciences
PR China

Birun Lin*
Plant Protection Research Institute
Guangdong Academy of Agricultural Science
Guangzhou 510640, PR China
E-mail: linbr@126.com

Xiangyang Gao
College of Life Science
South China Agricultural University
PR China

Huifang Shen
Plant Protection Research Institute
Guangdong Academy of Agricultural Science
PR China

*Corresponding author

Financial support: National Natural Science Foundation of China (No. 30471161) and the Guangdong Natural Science Foundation of China (No. 8151064001000010 and No.06025388).

Keywords: mutagen nitrogen ion beam implantation, Streptomyces griseovariabilis GAAS2057, wanlongmycin.

In an attempt to obtain an industrial strain with higher yield of wanlongmycin, the wild strain Streptomyces griseovariabilis GAAS2507 was mutated by a novel mutagen, nitrogen ion beam with energy of 20 kilo electron volts (KeV) and dose ranging from 7.80 x 10¹⁴ to 2.86 x 10¹⁵ ions/cm². One mutant strain WN939 was obtained. Its yield of wanlongmycin reached 271.24 µg/mL, which was 82.10% higher than that of the wild strain. The mutant strain WN939 was relatively stable for the production of wanlongmycin through six successive transfers of cultures and a repeat fermentation in a 30 L fermentor. In addition, the mutant strains were investigated and divided into five types by their colony phenotypes and production of wanlongmycin. Among them, three types mutant strains exhibited positive mutation, while the other two types mutant strains exhibited negative mutation.