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Figure
1. Plantlet regeneration of Tigridia pavonia (L.f) DC
from twin-scales.
(a)
Shoots (arrows) of 0.5-1 cm in length developing at the basal portion
of the scale, two weeks after culture in MS medium supplemented with
4.5 µM 2,4-dichlorophenoxiacetic acid (2,4-D) and 2.2
µM 6-benzilaminopurine (BAP).
(b)
Shoot proliferation after 8 weeks culture in N6 medium supplemented
with 4.5 µM of 2,4-D and 2.2 µM
of BAP.
(c)
Rooting of shoots, after 3 weeks on N6 basal medium supplemented
with 1 g l-1 activated
charcoal (AC) and 2.6 µM naphtalenacetic acid (NAA).
(d)
A regenerated plantlet after 16 weeks in culture.
(e)
Plants during acclimation 20 weeks after culture initiation.
(f)
ISSR profiles generated by the primer ASSR-15 using T. pavonia individuals
after two subcultures (lines 1-9) and from a mother plant grown under
greenhouse conditions (line 10). M, represents the DNA ladder. bp, base
pairs. |
