Table 1 - Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading

Table 1. Protein immobilization yield of large and small particle (LP and SP) size Sepharose 6B-CL immobilized penicillin G acylase at different protein loadings.

Biocatalyst	Loaded protein (g g_gel^-1)	Immobilized protein (g g_gel^-1)	Protein immobilization yield (%)
LP1	0.016	0.0154	97
LP2	0.035	0.0341	98
LP3	0.054	0.053	98
LP4	0.073	0.0691	95
LP5	0.092	0.0824	90
LP6	0.13	0.0912	70
SP1	0.016	0.0154	97
SP2	0.035	0.0341	98
SP3	0.054	0.0529	98
SP4	0.073	0.0698	96
SP5	0.092	0.0843	92
SP6	0.13	0.0893	69

Supported by UNESCO / MIRCEN network

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