|
| Table 2. Initial reaction rates (v) for free (FPGA) and immobilized (SP1) penicillin acylase at different penicillin G concentrations at pH 7.8 and 30ºC, and calculated kinetic parameters. In the case of SP1, kinetic parameters are intrinsic. |
FPGA |
SP1 |
||
[penicillin G] |
v (mmol min-1 gprot-1) |
[penicillin G] |
v (mmol/min·gprot) |
0.08 |
18.7 |
|
|
0.1 |
21.2 |
|
|
0.14 |
24.4 |
0.14 |
1.82 |
|
|
0.17 |
1.96 |
|
|
0.20 |
2.24 |
0.23 |
29.3 |
0.23 |
2.67 |
|
|
0.3 |
3.65 |
0.5 |
35.0 |
0.5 |
5.75 |
|
|
0.8 |
8.41 |
|
|
2 |
14.78 |
|
|
4 |
16.51 |
10 |
41.6 |
10 |
17.40 |
|
|
25 |
17.73 |
KM (mM) |
Vmax (mmol/min·gprot) |
KM (mM) |
Vmax (mmol/min·gprot) |
0.10 |
42.0 |
0.45 |
18.18 |
|
|
|