Table 3. Effect of internal diffusional restrictions on glyoxyl-agarose immobilized penicillin acylase of different particle size and protein loadings. LP: large particle size (average diameter 105.1 µm); SP small particle size (average diameter 51.1 µm); IP: immobilized protein in g/gbiocat; v: initial effective reaction rate in moles/min x gprotein; η: biocatalyst effectiveness factor; Vmax: intrinsic maximum reaction rate in mM/min; Φ: substrate Thiele modulus. Subscripts in v and η refer to penicillin concentration (mM). |
Biocatalyst |
IP |
v2 |
v10 |
v25 |
η2 |
η10 |
η25 |
Vmax |
φ |
LP1 |
0.0154 |
10.91 |
14.26 |
14.26 |
0.738 |
0.820 |
0.805 |
268.8 |
2.40 |
LP2 |
0.0341 |
5.32 |
11.02 |
13.11 |
0.360 |
0.633 |
0.740 |
595.1 |
3.57 |
LP3 |
0.0530 |
3.79 |
11.15 |
13.57 |
0.256 |
0.641 |
0.765 |
925.0 |
4.45 |
LP4 |
0.0691 |
2.72 |
9.85 |
13.23 |
0.184 |
0.566 |
0.746 |
1206.0 |
5.08 |
LP5 |
0.0824 |
2.28 |
8.44 |
12.19 |
0.154 |
0.485 |
0.688 |
1438.1 |
5.55 |
LP6 |
0.0912 |
2.27 |
7.89 |
10.59 |
0.153 |
0.453 |
0.597 |
1591.7 |
5.84 |
SP1 |
0.0154 |
10.79 |
12.91 |
13.88 |
0.730 |
0.742 |
0.783 |
268.8 |
1.17 |
SP2 |
0.0341 |
10.82 |
12.72 |
13.48 |
0.732 |
0.731 |
0.760 |
595.1 |
1.74 |
SP3 |
0.0529 |
6.98 |
11.15 |
13.31 |
0.472 |
0.641 |
0.751 |
923.3 |
2.16 |
SP4 |
0.0698 |
5.38 |
11.42 |
13.64 |
0.364 |
0.656 |
0.769 |
1218.2 |
2.48 |
SP5 |
0.0843 |
5.07 |
11.38 |
13.30 |
0.343 |
0.654 |
0.750 |
1471.3 |
2.73 |
SP6 |
0.0893 |
4.21 |
10.53 |
11.90 |
0.285 |
0.605 |
0.671 |
1558.5 |
2.81 |
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