Table 4. Inhibition of Vibrio harveyi PSU 2015 by 0.45 µm and 0.22 µm culture filtrates from Pseudomonas sp. W3.

Treatment

Inhibition zone (mm)

0.45 µm culture filtrate

0.22 µm culture filtrate

Enzyme

 Control

17.8 ± 0.3^a

15.2 ± 0.4^a

 Proteinase K

13.8 ± 0.2^bc

14.7 ± 0.7^abc

 Pronase

13.9 ± 0.4^bc

14.4 ± 0.5^abc

 Pepsin

14.4 ± 0.2^bc

14.3 ± 0.2^abc

 α-chymotrypsin

13.0 ± 0.2^c

14.0 ± 0.5^c

 Trypsin

14.0 ± 0.6^bc

15.1 ± 0.7^ab

 Lysozyme

14.5 ± 0.2^bc

14.8 ± 0.2^abc

 Lipase

14.8 ± 0.4^b

14.4 ± 0.4^abc

 α-amylase

15.0 ± 0.4^b

14.0 ± 0.4^bc

 Temperature of incubation (ºC)

 Control (30)

18.0 ± 0.5^a

15.4 ± 0.4^a

 50

17.5 ± 0.5^a

15.3 ± 0.3^a

 65

16.5 ± 0.4^b

14.7 ± 0.1^ab

 75

15.9 ± 0.5^b

15.0 ± 0.4^ab

 100

16.2 ± 0.3^b

14.7 ± 0.4^ab

 Autoclave (121ºC, 15 min)

15.1 ± 0.4^c

14.7 ± 0.2^ab

 Autoclave (121ºC, 30 min)

14.3 ± 0.2^d

14.0 ± 0.7^b

pH

 Control (pH 7.40)

17.1 ± 0.5^a

16.8 ± 0.7^a

 1

5.3 ± 0.5^f

10.1 ± 0.7^c

 2

13.1 ± 0.4^cd

15.5 ± 0.7^b

 3

13.3 ± 0.5^c

15.4 ± 0.5^b

 4

13.9 ± 0.5^bc

16.1 ± 0.3^ab

 5

14.4 ± 0.5^bc

15.6 ± 0.8^ab

 6

16.5 ± 0.3^b

16.0 ± 0.5^ab

 7

16.3 ± 0.3^b

15.5 ± 0.8^ab

 8

12.4 ± 0.4^d

15.5 ± 0.3^ab

 9

12.2 ± 0.4^d

14.9 ± 0.6^b

 10

11.1 ± 0.4^e

14.9 ± 0.8^b