Table 2. Effect of BAP and AS concentration on phenolic oxidation and average number of shoots, roots and leaves induced from embryogenic axesofcommon bean (Phaseolus vulgaris L.) regardless of the genotype. Data were recorded after 30 days of culture on induction medium supplemented with different BAP and AS concentrations. |
Treatments |
|
|
|
|
|
BAP (mgl-1) |
AS (mgl-1) |
Shoots1 |
Leaves1 |
Roots1 |
Phenolic oxidation1 |
0 |
0 |
0.00 ± 0.002 d |
0.89 ± 0.05 a |
3.53 ± 0.18 a |
0.00 ± 0.00 e |
0 |
20 |
0.00 ± 0.00 d |
0.34 ± 0.04 d |
1.85 ± 0.16 b |
0.19 ± 0.03 c d |
0 |
40 |
0.37 ± 0.04 b c |
0.81 ± 0.07 a b |
0.65 ± 0.05 c |
0.45 ± 0.04 a |
5 |
0 |
0.09 ± 0.04 d |
0.00 ± 0.00 e |
0.09 ± 0.04 d |
0.00 ± 0.00 e |
5 |
20 |
0.56 ± 0.08 a b |
0.70 ± 0.13 a b c |
0.01 ± 0.01 d |
0.31 ± 0.04 b c |
5 |
40 |
0.66 ± 0.09 a |
0.49 ± 0.09 c d |
0.00 ± 0.00 d |
0.05 ± 0.02 d e |
10 |
0 |
0.01 ± 0.01 d |
0.00 ± 0.00 e |
0.00 ± 0.00 d |
0.31 ± 0.04 b c |
10 |
20 |
0.20 ± 0.06 c d |
0.25 ± 0.06 d e |
0.31 ± 0.08 c d |
0.43 ± 0.04 a b |
10 |
40 |
0.40 ± 0.05 b c |
0.51 ± 0.08 b c d |
0.00 ± 0.00 d |
0.15 ± 0.02 d |
1 Values represent the average shoot, leaves and roots formation per
treatment in 15 Petri dishes with 10 explants and three replicates. |
|