Table 1. Quantitative potency assay for pVGI.1(VEGF2) produced in different hosts and stability testing. |
Experiment |
Host |
Treatment |
DNA Concentration by A260 (mg/ml) |
Potency Value Calculated by SoftMax Pro® (mg/ml) |
Change
in Response |
1 |
DH5a |
Untreated |
33 |
30 |
- |
DH5a |
Heat treated (90ºC for 8 hrs) |
33 |
9 |
Reduction in upper asymptote |
|
DH5a |
Sheared (22 gauge needle for 30 times) |
33 |
11 |
Reduction in upper asymptote (ratio of standard/sample changed from 1 to 1.3)) |
|
2 |
DH5a |
Untreated |
1000 |
1000 |
- |
Non-DH5a host |
Untreated |
1000 |
780 |
Change in parameter B |
|