Table 1. Quantitative potency assay for pVGI.1(VEGF2) produced in different hosts and stability testing.



Experiment

Host

Treatment

DNA Concentration by A260 (mg/ml)

Potency Value Calculated by SoftMax Pro® (mg/ml)

Change in Response
Curve
(4 PL Parameters)

1

DH5a

Untreated

33

30

-

DH5a

Heat treated

(90ºC for 8 hrs)

33

9

Reduction in upper asymptote
(ratio of standard/sample changed from 1 to 1.3)

DH5a

Sheared

(22 gauge needle for 30 times)

33

11

Reduction in upper asymptote (ratio of standard/sample changed from 1 to 1.3))

2

DH5a

Untreated

1000

1000

-

Non-DH5a host

Untreated

1000

780

Change in parameter B
(change from 3.383 ± 1.066 to 17.521)


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