Plant Biotechnology
 

Microbial Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 13 No. 2, Issue of March 15, 2010
© 2010 by Pontificia Universidad Católica de Valparaíso -- Chile Received May 12, 2009 / Accepted October 23, 2009
DOI: 10.2225/vol13-issue2-fulltext-2
RESEARCH ARTICLE

Optimization of worm-bed leachate for culturing of tomato (Lycopersicon esculentum Mill) inoculated with Glomus fasciculatum and Pseudomonas fluorescens

María Ángela Oliva-Llaven
Facultad de Medicina Veterinaria y Zootecnia
Universidad Autónoma de Chiapas
Chiapas, México

Ludwi Rodríguez-Hernández
Instituto Tecnológico de Tuxtla Gutiérrez
Chiapas, México 

Paula Mendoza-Nazar
Facultad de Medicina Veterinaria y Zootecnia
Universidad Autónoma de Chiapas
Chiapas, México

Benigno Ruiz-Sesma
Facultad de Medicina Veterinaria y Zootecnia
Universidad Autónoma de Chiapas
Chiapas, México

José David Álvarez-Solís*
Departamento de Agroecología
Colegio de la Frontera Sur
Chiapas, México
E-mail: dalvarez@ecosur.mx

Luc Dendooven
Laboratorio de Ecología de Suelos
Departamento de Biotecnología y Bioingeniería
Centro de Investigación y de Estudios Avanzados
Instituto Politécnico Nacional
México

Federico A. Gutiérrez-Miceli
División de Posgrado e Investigación
Instituto Tecnologico de Tuxtla Gutiérrez
Chiapas, México

*Corresponding author

Keywords: Glomus fasciculatum, organic cultivation of tomato, pH in tomato fruit, soluble solids.

Abbreviations:

FAWBL: frequency of application of worm-bed leachate
RSM: response surface methodologies
WBL: worm-bed leachate

Abstract   Full Text

A response surface technique was used to analyze the effect of Glomus fasciculatum, Pseudomonas fluorescens and worm-bed leachate (WBL) on growth, yield and characteristics of tomato (Lycopersicon esculentum Mill). The treatments combined inoculation with or without P. fluorescensor G. fasciculatum and the application of WBL at 20% (v/v) each day or every three days. Plant height, number of leaves and yield of tomato fruits was not affected by the factors studied. However, plants with foliar application of WBL each day developed wider stems than those with an application every three days. The pH of the fruits was lower when WBL was applied every three days compared to a daily application. The soluble solids content of the fruits was higher when WBL was applied daily compared to those sprayed every three days. Plant development was not affected by addition of P. fluorescens, G. fasciculatum or WBL, but WBL changed fruit characteristics.

Supported by UNESCO / MIRCEN network