Table 1. Parameters evaluated for the adaptation of AFLPs® in C. vulgaris.

Step

Parameter

variants

 A
 (Restriction digestion)

 Duration

3

 Temperature

4

 Buffer system

3

 Enzyme distributor

3

 Enzyme proportions

20

 B
 (Ligation)

 Duration

3

 Temperature

3

 Buffer system

3

 Enzyme distributor

3

 C
 (PCR amplification)

 Enzyme distributor

3

 distributor of IRDye oligos

3

 Oligo proportions

5

 Dilutions

5

 D
 (Separation and
 Visualization)

 Gel matrix solution

2

 Loading dye

2

 Buffer composition

2