Figure 1. Comparison of the
ability in regeneration of P. yezoensis protoplasts prepared by the
treatment with AMX solution and allantoin. Scale bars: 20 µm.
(a)
Protoplast prepared from gametophytic blades by treatment with AMX solution. No
regeneration was observed.
(b)
Protoplast prepared from gametophytic blades which were cultured in the ESL
medium containing 10 mM allantoin for 3 weeks.
(c)
Ability of regeneration in protoplasts prepared from allantoin-treated gametophytic
blade. |