|
| Table 3. Peanut microsatellite markers with good amplification quality in Luhua- 14. |
Primer |
Forward primer (5'-3') |
Reverse primer (5'-3') |
Motif |
Annealing Temperature (ºC) |
Expected Size (bp) |
|
X |
Y |
|||||
F2 |
GGATTCATTCGAGGGTGG |
AAGGCATCTTTGTCGTTG |
(CTT)6 |
58 |
56 |
168 |
F3 |
CAGCAACTTTCTTCAACC |
CTCTGAATCTCGCACCCT |
(AGA)6 |
58 |
56 |
108 |
F9 |
TTCGAGCTTGAGGAAGAG |
AACGGAATTTGAGATGATAG |
(AATC)6 |
56 |
54 |
191 |
F13 |
ATCCTACGATTTCTTTACTG |
TCGATGGAGGGATGATTT |
(CT)25 |
50 |
48 |
152 |
H2 |
TCCCAGAACAACAATCAC |
AGAAGAATACGAGCACCA |
(TCT)5 |
54 |
52 |
215 |
H4 |
TCTTGATCTTATCTTCCCTG |
CAAATGTGGTGTCGCTGA |
(TCA)5 |
56 |
54 |
244 |
H6 |
CCAACTCGACTCGGCTTTC |
TTGCTCCTCTAACAACCTTTCT |
(TC)7 |
56 |
54 |
221 |
H7 |
CCTCACCAACCAGGATAC |
TTTCGTCTTTGCAGTTTC |
(CTT)4 |
54 |
52 |
122 |
H8 |
ATCAAGCCATAATATGTTCC |
CAACCCAAGCACCTCTAC |
(TA)6 |
54 |
52 |
193 |
H10 |
AGAGTAAGGGTGCTTCTG |
ATGCTTCTCCCATTTCCA |
(GAT)5 |
54 |
52 |
142 |
H11 |
CCGAACTCAACCCAAACA |
AGCTCCGGCAAGAAAGAA |
(CAC)4 |
56 |
54 |
178 |
H13 |
GCTTTTATTTCACATACATCAC |
GGTAGGGCACTGTTCACTT |
(CTT)7 |
54 |
52 |
165 |
H14 |
ACGAAGCCTAAGAACGGAAAG |
ATGCCACCAGCAAAGGAG |
(AC)5 |
56 |
54 |
258 |
H15 |
CGGTCGTAGTAGTTGTGG |
GAGGCTTAAAGGAAGTGG |
(CT)6 |
56 |
54 |
216 |
PD10 |
ACTTGCTTTGGATGCTTG |
CTTTTCTTTTCCCGGACT |
(TTA)7 |
50 |
48 |
208 |
PD11 |
CTCTTTCATTATTGGGTTTG |
AGATTGTGCAGCCTCATC |
(TAA)7 |
54 |
52 |
152 |
PD15 |
ACTAACACCATTTCCGACAG |
TGGGAGCCTATGAAACCT |
(TTC)5 |
58 |
56 |
163 |
PD17 |
CTTTCCCATATTCCTTCGT |
TGTTCCTCCTCCTGCTTT |
(TCT)6 |
56 |
54 |
129 |
PD18 |
ATCATCATCATCATCGTC |
AGTTTCTGGTACTGTCGC |
(TCA)6 |
54 |
52 |
152 |
PD19 |
CGTGGAGAACGTGCTTAG |
CCATAGATCGAAATAGGTAG |
(TTA)5 |
54 |
52 |
210 |
PD20 |
GAAGAAAATAGCAGGACT |
CGTAGTATCTTGATAACCC |
(CTT)6 |
56 |
54 |
254 |
PD23 |
TACAATGTTGCCTTTCAC |
CAACTCCTCACTACTCCC |
(TAA)6 |
56 |
54 |
205 |
PD28 |
ATTAAAGATGGCTTTGGAG |
AGAGTAGTGGCAGTGACCT |
(AAG)6 |
54 |
52 |
240 |
PD33 |
GGGGAACATGGTCTCATC |
GGGCTTTCTCATAGGTGG |
(TCT)5 |
60 |
58 |
139 |
PD35 |
GAACCTTAGAGCTTGTGG |
GACGAGTAATTCTCCTTT |
(TCA)5 |
50 |
48 |
194 |
PD36 |
GCATCTACCATTGGAGTG |
ACAAAGCAAAGCGGATAC |
(TAC)5 |
56 |
54 |
122 |
PD37 |
GTGAGAATGGAGACTTTG |
TCACCATCACTTTATCAG |
(TAA)5 |
54 |
52 |
149 |
PD38 |
GGGAGTGAAGTTAAAGAA |
TGAATCCAACACCGTATC |
(GCT)5 |
54 |
52 |
140 |
PD40 |
CTAGTACTTTTCTAAATTGGAC |
TAATGGTTTTGGGTCACA |
(GAA)5 |
54 |
52 |
108 |
PD49 |
CAACTGGTCCCAATAATCC |
GCTATGCCTCCTTCTACAAAA |
(ACA)5 |
58 |
56 |
135 |
PD52 |
CAGTTGAAATGCAAGGGTC |
TGGAGTATCCAGGTGAGG |
(AAC)5 |
58 |
56 |
189 |
PD54 |
CTCTTCTTCTGCCTCTGC |
AGTAGCAACAACCCTCAT |
(TCAA)5 |
56 |
54 |
202 |
PD59 |
ACCCTTTCACCCTCTTCA |
AATGTGGCTGCTCTGTTA |
(TCT)8 |
56 |
54 |
130 |
|
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