Figure 1. Plasmid construction of the pCAMBIA1301 binary vector used for transformation of Italian ryegrass plants. HPT, hygromycin phosphotransferase; int-GUS, β-glucuronidase containing intron; 35S, cauliflower mosaic virus 35S promoter; T35S, polyA cauliflower mosaic virus 35S terminator; TNOS, polyA  nopaline synthase terminator; Bold bar indicates the region amplified by PCR. LB andRB indicates leaf and right border, respectively.