Figure 3. Regeneration of transgenic plants and histological GUS assay of putatively transgenic calli of seven Korean Italian ryegrass cultivars.
(A) Regeneration of putatively transformed plants of Hanswan 101 after 3 weeks culture on selection medium containing 50 mg/L hygromycin.
(B) Histochemical GUS activity in leaf tissue in Hanswan 101 after 5 days co-culture with Agrobacterium.
(C) Histochemical GUS activity in leaf tissue in Korwinmaster after 5 days co-culture with Agrobacterium.
(D) Hygromycin-resistant calli showing GUS gene expression of seven Korean Italian ryegrass cultivars.
(E) Rooted transformants ready to be transferred to the greenhouse.
(F) Transgenic plants grown in pots in the green house.


Supported by UNESCO / MIRCEN network