Figure 3. Regeneration of transgenic
plants and histological GUS assay of putatively transgenic calli of seven Korean Italian ryegrass
cultivars.
(A) Regeneration of putatively transformed plants of
Hanswan 101 after 3 weeks culture on selection medium containing 50 mg/L
hygromycin.
(B) Histochemical GUS activity in leaf tissue in
Hanswan 101 after 5 days co-culture with Agrobacterium.
(C) Histochemical GUS activity in leaf tissue in
Korwinmaster after 5 days co-culture with Agrobacterium.
(D) Hygromycin-resistant
calli showing GUS gene expression of seven Korean Italian ryegrass cultivars.
(E) Rooted transformants ready to be transferred to
the greenhouse.
(F) Transgenic
plants grown in pots in the green house.
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