Table 2. Protein concentration and hrIDS biological activity (nmol h-1 mg-1 of total protein).


Fraction

Protein concentration
(mg ml-1)

hrIDS activty
(nmol h-1 mg-1 of total protein)

Supernatant

3.93

1.20

 >100 kDa (F1)

3.78

1.19

30-100 kDa (F2)

2.81

1.86

<30 kDa (F3)

3.59

0.40

Cell lysate

1.55

2.82

Values of hrIDS activty are represented as nmol of transform substrate (4-methylumbelliferyl-α-iduronate 2-sulphate)/ mg of total protein, (Voznyi et al. 2001).

Supported by UNESCO / MIRCEN network