Process Biotechnology
  Microbial Biotechnology
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 13 No. 4, Issue of July 15, 2010
© 2010 by Pontificia Universidad Católica de Valparaíso -- Chile Received September 29, 2009 / Accepted February 25, 2010
DOI: 10.2225/vol13-issue4-fulltext-6
REPORT

Kinetic characteristics of β-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26

Benjamas Cheirsilp*
Department of Industrial Biotechnology
Faculty of Agro-Industry
Prince of Songkla University
Hat-Yai, 90112, Thailand
E-mail: benjamas.che@psu.ac.th 

Suleeporn Kitcha
Department of Industrial Biotechnology
Faculty of Agro-Industry
Prince of Songkla University
Hat-Yai, 90112, Thailand 

Supasilp Maneerat
Department of Industrial Biotechnology
Faculty of Agro-Industry
Prince of Songkla University
Hat-Yai, 90112, Thailand

*Corresponding author

Financial support: Thai Government and Prince of Songkla University in the fiscal year of 2006-2008 under Grant AGR5011990074S.

Keywords: Bacillus subtilis ZK8, cell adsorption, jiean- peptide, response surface methodology, wood chips.

Abbreviations:

CD: cyclodextrin
CGTase: cyclodextrin glycosyltransferase
DP: degree of polymerization

Abstract   Full Text

The kinetic characteristics of β-cyclodextrin production by a cyclodextrin glycosyltransferase (CGTase) produced by Bacillus sp. C26, a new isolate from a soil sample was investigated. Considering highest yield and initial production rate of β-cyclodextrin, among the starches examined, soluble starch, tapioca starch, sago starch, corn starch and rice starch, tapioca starch was the best substrate for this CGTase. The optimum temperature for tapioca starch gelatinization prior to its use as a substrate for β-cyclodextrin production was 65ºC. The yield and initial production rate of β-cyclodextrin increased with increasing starch concentration up to 6% and an enzyme concentration up to 48 U/g-starch. The kinetic parameters of Vmax and Km of β-cyclodextrin production from tapioca starch by CGTase were 1.59 mg/mL/h and 22.3 mg/mL, respectively. Considering high initial production rate and high yield of β-cyclodextrin, the optimum reaction temperature was at 50ºC. This study provided the necessary kinetic information that may be useful to define the most suitable condition for industrialized production ofβ-cyclodextrin with the high yield and productivity.

Supported by UNESCO / MIRCEN network