Figure 1. Time course of biomass, X (●), glycerol, Gly (+), citric acid, CA (■), isocitric acid (□), erythritol, ER (▲), and mannitol, MAN (∆), during fed-batch fermentation by Y. lipolytica Wratislavia 1.31 (a) and Y. lipolytica Wratislavia AWG7 (b) strain growing on crude glycerol (BDK Biodiesel GmBH, Kyritz, Germany).
Cultivation conditions: culture broth medium was fed with two identical portions of glycerol after 24 and 48 hrs of cultivation in order to obtain a final glycerol concentration of 200 g∙L^-1.
Data are the average of duplicate cultures. Error bars indicate the standard deviations of mean data values.