Figure 4. The factors affecting the efficiency of Capsicum chinense genetic transformation. The effect of different factors on the transformation efficiency was analyzed. (a) The leaf explants from four-week-old plantlets were co-cultivated with the A. tumefaciens strain that was previously cultivated in the presence of different concentrations of acetosyringone, as described. (b) The leaf explants from the pepper plantlets with different ages were transformed under the optimal conditions described in the text. (c) The leaf explants from four-week-old plantlets were pre-incubated in MS medium for several periods before co-cultivation.


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