Figure 5. The detection of transgene transcripts in the transformed pepper leaves. Two micrograms of total RNA was extracted from transformed leaves and was reverse transcribed and PCR amplified with gene-specific primers. Two-microlitre aliquots of each RT-PCR product were fractionated by agarose gel electrophoresis and stained with 1 µg·mL^-1 ethidium bromide. (a) RT-PCR amplification of the GUS reporter transcript; (b) RT-PCR amplification of the PR10 and esterase transcripts. M: 1 Kb DNA ladder (Invitrogen); U: untransformed leaves; Mex: empty pCAMex binary vector; 2301: empty pCAMBIA2301 binary vector; PR10: pCAMex::PR10 construct; Est: pCAMex::Esterase construct; (+) symbol: PCR positive control using pCAMBIA2301 binary vector as template.