Table 3. Experimental results of the path of steepest ascent (descent).


 

X2a
 (SMH, %, v/v)

X4
(MgSO4·7H2O, %, w/v)

Jiean-peptide
(mg/l)

Base point (zero level in the Plackett–Burman design)

15

0.70

 

Slope (estimated coefficient ratio from equation)

39.06

32.86

 

New step unit

39.06 x 0.05b = 2

32.86 x 0.0025c = 0.08

 

Experiment number 1

15

0.70

228.42 ± 30.68

Experiment number 2

17

0.62

243.67 ± 15.89

Experiment number 3

19

0.54

299.06 ± 32.99

Experiment number 4

21

0.46

317.49 ± 22.11

Experiment number 5

23

0.38

371.36 ± 32.79

Experiment number 6

25

0.30

331.42 ± 42.31

Experiment number 7

27

0.22

310.65 ± 45.12

Experiment number 8

29

0.14

262.78 ± 31.04

Experiment number 9

31

0.06

271.36 ± 27.75

a The symbols were the same those in Table 1.
0.05 b and 0.0025c are the factors determined by experimenter based on process knowledge or other practical consideration.

Supported by UNESCO / MIRCEN network