Table 4. Design and responses of the central composite design (CCD).



Run

Coded values and real values

Jiean-peptide (mg/l)

 

X2a (SMH, %, v/v)

X4 (MgSO4·7H2O, %, w/v)

Experimental

Predicted

1

-1 (18)

-1 (0.28)

258.50 ± 7.45

263.12

2

-1 (18)

1 (0.48)

209.43 ± 22.66

233.29

3

1 (28)

-1 (0.28)

285.27 ± 45.55

277.01

4

1 (28)

1 (0.48)

303.99 ± 18.42

314.96

5

-1.41 (16)

0 (0.38)

280.26 ± 12.98

264.02

6

1.41 (30)

0 (0.38)

329.60 ± 26.48

368.43

7

0 (23)

-1.41 (0.24)

238.36 ± 20.67

245.04

8

0 (23)

1.41 (0.52)

271.31 ± 33.11

250.77

9

0 (23)

0 (0.38)

406.87 ± 3.75

391.94

10

0 (23)

0 (0.38)

387.62 ± 24.64

391.94

11

0 (23)

0 (0.38)

382.43 ± 24.97

391.94

12

0 (23)

0 (0.38)

382.43 ± 41.08

391.94

13

0 (23)

0 (0.38)

400.33 ± 34.24

391.94

a The symbols were the same those in Table 1.


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