Table 4. Design and responses of the central composite design (CCD). |
Run |
Coded values and real values |
Jiean-peptide (mg/l) |
||
|
X2a (SMH, %, v/v) |
X4 (MgSO4·7H2O, %, w/v) |
Experimental |
Predicted |
1 |
-1 (18) |
-1 (0.28) |
258.50 ± 7.45 |
263.12 |
2 |
-1 (18) |
1 (0.48) |
209.43 ± 22.66 |
233.29 |
3 |
1 (28) |
-1 (0.28) |
285.27 ± 45.55 |
277.01 |
4 |
1 (28) |
1 (0.48) |
303.99 ± 18.42 |
314.96 |
5 |
-1.41 (16) |
0 (0.38) |
280.26 ± 12.98 |
264.02 |
6 |
1.41 (30) |
0 (0.38) |
329.60 ± 26.48 |
368.43 |
7 |
0 (23) |
-1.41 (0.24) |
238.36 ± 20.67 |
245.04 |
8 |
0 (23) |
1.41 (0.52) |
271.31 ± 33.11 |
250.77 |
9 |
0 (23) |
0 (0.38) |
406.87 ± 3.75 |
391.94 |
10 |
0 (23) |
0 (0.38) |
387.62 ± 24.64 |
391.94 |
11 |
0 (23) |
0 (0.38) |
382.43 ± 24.97 |
391.94 |
12 |
0 (23) |
0 (0.38) |
382.43 ± 41.08 |
391.94 |
13 |
0 (23) |
0 (0.38) |
400.33 ± 34.24 |
391.94 |
a The symbols were the same those in Table 1. |
|