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Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut Chuan Tang Wang* Shan Lin Yu Shu Wei Zhang Xiu Zhen Wang Yue Yi Tang Jian Cheng Zhang Dian Xu Chen *Corresponding author Financial support: The authors thank support from the earmarked fund for Modern Agro-industry Technology Research System (MATRS) Peanut Program (Grant No. nycytx-19), Ministry of Agriculture, China, China Natural Science Foundation (Grant No. 30300224), 863 New and High Technology Project (Grant No. 2006AA10A114), New and High Technology Innovation Foundation of Shandong Academy of Agricultural Sciences (Grant No. 2006 YCX013), and Young Scientists Foundation of Shandong Academy of Agricultural Sciences (Grant No.2007YQN007). Keywords: identification, NIRS, PCR, peanut, true hybrid.
A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut. |