Figure 5. Wound induction of PStSN1 activity in transgenic Arabidopsis lines. For mechanical wounding treatments, leaves were gently pressed with a pair of sterilized serrated forceps. Four independent transgenic Arabidopsis lines harboring PStSN1::GUS construct (Lines 2B, 5C, 8B and 11A) and one 35S::GUS transgenic control line were evaluated.
(a) Representative leaves of weak expressing line (Line 11A) were stained at 1, 2 or 3 hrs after wounding (note that leaves were pressed one, two or three times, respectively).
(b) GUS activity assays of whole plants collected 2 hrs after wounding. Activity of the wounded plants was measured and relatively expressed to the average activity of the unwounded plants of the same line. Bar graphs represent the average percentage of GUS activity ± SE measured in ten biological replicates.
(c) Wound-inducible Ap-2 gene was amplified by RT-PCR (left panel) to check treatment effectiveness. Specific Actin-2 transcript amplification was detected in all plants as an internal control for cDNA synthesis (right panel). M: Molecular Marker 1 Kb; (-) Negative PCR control (without DNA). * denote significant differences (P < 0.05).