Figure 6. Constitutive PStSN1 activity Arabidopsis lines after phytohormones, light, UV radiation or Pseudomonas syringae infection. Independent transgenic Arabidopsis lines harboring PStSN1::GUS constructs (Lines 2B, 5C, 8B and 11A) and a 35S::GUS transgenic line were evaluated upon different treatments. GUS activity was measured and percentages were calculated relative to untreated control data. The data represent the average percentage of GUS activity ± SE measured in eight (a and b) or ten biological replicates (d).
(a) Phytohormones Treatments. Whole plants were sprayed with abscisic acid (ABA), indol acetic acid (IAA), gibberellic acid, (GA₃) or mock-treated with water and collected al 6 h to evaluate GUS activity.
(b) Light Treatment. Plants grown under 10 hrs dark-14 hrs light cycle, were covered and kept in darkness or left to continue the normal light cycle for 4 h prior to collection.
(c) UV radiation Treatment. Fifty 14-days-old seedlings of each PStSN1::GUS line were collected 2 hrs after exposure to a UV light bulb and pooled (Errors bars correspond to three techniques replicates).
(d) Pseudomonas syringae infection. Pathogen Pseudomonas syringae suspensions (Infected) or 10 mM MgSO₄ (Control) were sprayed on intact leaves and whole plants were collected at 72 hrs post infection.
(e) Treatment effectiveness was assayed by RT-PCR of different inducible genes: ABA-inducible RD22 (NM_122472); IAA-inducible SAURAC1 (S70188), GA₃-inducible APT1 (NM_179383) and light-inducible CHS gene (NM_121396). Specific Actin-2 (NM_112764) transcript amplification was detected in all plants as an internal control of cDNA synthesis (lower panel of each treatment). M: Molecular Marker 1 Kb; (-) Negative PCR control (without DNA).