Figure 1. Scheme of pEglABglA construction.
1. PCR amplification of BglA gene.
2. Digestion (BamHI/PstI) of BglA Amplicon.
3. Digestion (BamHI/PstI) of pEglA vector.
4. Ligation of BglA amplicon into BamHI/PstI site of pEglA.