^a E. coli DH5α is the parental strain. E. coli DH5α (pBglA) and E. coli DH5α (pEglA) express BglA and EglA enzymes, respectively. E. coli DH5α (pEglABglA) express both EglA and BglA enzymes.
^b Specific activities of BglA and EglA were defined as the amount (nmoles) of p-nitrophenol (PNP) or Remazol Brilliant Blue (RBB) released, respectively, per μg protein extract per hour (pH 7, 85ºC for BglA and 55ºC for EglA). Means were calculated for three (BglA) and five (EglA) replicates.
^c Values followed by the same letters in the columns are not significantly different at 5% probability level. Means were calculated for three replicates.
^d Reducing sugars were analyzed by the Somogy-Nelson method, using supernatants of MM CMC medium after the growth of the E. coli strains.
^e Rheological analysis were carried out with rotational viscosimeter to determine the viscosity of MM CMC medium after the growth of the E. coli strains.
^f Nd: not determined.
* The values in the column are significantly different at 5% probability level.