Table 1. Media composition.

Medium

Composition

CIM (callus inducing
medium)

 1/2MS + 6-BA 0.5mg l^-1 + 2,4-D 0.1 mg l^-1 + sucrose 3% + agar 0.7%

CCM (co-cultivation
medium)

 1/2MS + 6-BA 0.5 mg l^-1 + 2,4-D 0.1 mg l^-1 + sucrose 3% + agar 0.7%
 + AS (0-125) μmol l^-1

RM (recovery
medium)

 1/2MS + 6-BA 0.5 mg l^-1 + 2,4-D 0.1 mg l^-1 + sucrose 3% + agar 0.7%
 + Cef 400 mg l^-1

CIM-CK

 1/2MS + 6-BA 0.5 mg l^-1 + 2,4-D 0.1 mg l^-1 + sucrose 3% + agar 0.7%
 + Cef 400 mg l^-1 + kan (100/125) mg l^-1

SIM (shoot inducing
medium)

 1/2MS + 6-BA 1.0 mg l^-1 + 2,4-D 0.1 mg l^-1+ sucrose 3% + agar 0.7%
 + Cef 400 mg l^-1 + kan (100/125) mg l^-1

RIM (root inducing
medium)

 1/2MS + 2,4-D 0.5 mg l^-1 + sucrose 3% + agar 0.7% + Cef 400 mg l^-1