|
| Table 2. Thirty G. arboreum ESTs showing amplified size of the amplicons, the number of SNPs detected for each EST, and their sequence and position in the amplicon. |
Sr # |
Best Blast homology |
Conserved region |
Amplicons size |
No. of SNPs/ |
SNP/ Indel location/ position |
1 |
Histone H2B-3 |
350-650 |
300 |
2 |
+A (595) |
2 |
NADPH-cytochrome P450 reductase |
450-550 |
100 |
0 |
- |
3 |
Werewolf |
201-570 |
400 |
1 |
- G (562) |
4 |
putative ATP binding protein |
30-150 |
120 |
2 |
T→C (30) |
5 |
common regulatory factor |
262-562 |
300 |
1 |
A→G (558) |
6 |
Photolyase/blue light photoreceptor PHR2 |
20-200 |
180 |
0 |
- |
7 |
S-adenosyl-L-homocysteine hydrolase |
246-663 |
420 |
3 |
C→A (249) |
8 |
Mitochondrial small heat shock protein |
139-440 |
300 |
0 |
- |
9 |
S-adenosyl methionine synthetase |
193-332 |
120 |
1 |
G→A (229) |
10 |
Histone H2B1 |
190-500 |
310 |
0 |
- |
11 |
Actin-depolymerizing factor 2 |
300-500 |
200 |
0 |
- |
12 |
CONSTANS-like protein 1 |
162-440 |
280 |
1 |
+A (178) |
13 |
Profilin |
35-540 |
500 |
0 |
- |
14 |
60S ribosomal protein L24 |
134-441 |
300 |
1 |
C→G (89) |
15 |
Similar to ATP-citrate-lyase |
79-424 |
350 |
0 |
- |
16 |
Contains similarity to protein kinase~gene |
10-450 |
440 |
1 |
-A (12) |
17 |
putative transcription factor BHLH6 |
111-296 |
200 |
1 |
-T (159) |
18 |
60S ribosomal protein L4/L1 |
169-346 |
200 |
0 |
- |
19 |
Histone H3.3 |
169-392 |
225 |
0 |
0 |
20 |
Luminal binding protein (BiP) |
46-411 |
365 |
2 |
A→C (49) |
21 |
Cystein proteinase |
267-356 |
100 |
0 |
0 |
22 |
Outer membrane lipoprotein – like |
40-428 |
400 |
0 |
0 |
23 |
14-3-3-like protein |
275-560 |
300 |
0 |
- |
24 |
Nucleotide-binding protein |
148-230 |
200 |
1 |
G→C (204) |
25 |
RAD23 protein |
23-105 |
80 |
0 |
- |
26 |
Elongation factor 1-gamma |
848-1010 |
160 |
3 |
G→C (899) |
27 |
E2, Ubiquitin-conjugating enzyme, putative |
10-378 |
370 |
0 |
- |
28 |
Beta-galactosidase-1 |
151-380 |
240 |
2 |
C→A (379) |
29 |
Alcohol dehydrogenase |
299-476 |
200 |
2 |
G→ T (431) |
30 |
vacuolar H+-ATPase subunit E |
70-214 |
144 |
3 |
G→A (137) |
|
|
|