Figure 4. Chromatographic purification of transiently expressed TproK.
(a) Octyl Sepharose chromatography.
(b) Q-Sepharose chromatography.
(c) Benzamidine Sepharose affinity chromatography.
(d) SDS-PAGE and Western blotting.
Lane 1: protein markers; Lane 2 and 3: concentrated spent medium and purified TproK; Lane 4: Western Bolt.