Plant Biotechnology
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 13 No. 6, Issue of November 15, 2010
© 2010 by Pontificia Universidad Católica de Valparaíso -- Chile Received July 1, 2010 / Accepted August 23, 2010
DOI: 10.2225/vol13-issue6-fulltext-10  

Characterization of the genetic structure of mango ginger (Curcuma amada Roxb.) from Myanmar in farm and genebank collection by the neutral and functional genomic markers

Shakeel Ahmad Jatoi*1,2· Akira Kikuchi1 · Dawood Ahmad1,3 · Kazuo N. Watanabe1

1Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
2Plant Genetic Resources Program, National Agricultural Research Center, Islamabad, Pakistan
3Institute of Biotechnology and Genetic Engineering, NWFP Agricultural University, Peshawar, Pakistan

*Corresponding author:

Financial support: The research work was supported by Grant-in-Aid # 21405017 from Japan Society for Promotion of Science, and Peace Nakajima Foundation.

Keywords: Curcuma amada, genetic diversity, mango-ginger, Myanmar, on-farm.

Abstract   Full Text

A preliminary characterization was undertaken to describe genetic structure of mango ginger (Curcuma amada) acquired from farmers and ex situ genebank in Myanmar using neutral (rice SSR based RAPDs) and functional genomic (P450 based analog) markers. The high polymorphism (> 91%) depicted has displayed existence of genetic variability in the germplasm investigated. Large number of source-specific alleles (neutral-markers = 78, functional-markers = 63) was amplified which revealed that neutral regions of the mango ginger were more variable compared with the functional regions. The major fraction of the molecular variance (neutral-markers = 85%, functional-markers = 93%) was explained within germplasm acquisition sources and this tendency was also supported by the estimate of gene diversity. The genebank accessions have shown comparatively more genetic variability than farmers’ accessions. The variability observed in mango ginger may possibly be associated with the long history of its cultivation under diverse ecological conditions. The two marker systems elucidated their high resolving power which detected variability even in fewer genotypes assayed. As the target sites of these markers are different, therefore, the variability detected is believed to cover diverse part of the genome together with neutral and functional regions. We found the concurrent use of the different types of molecular markers valuable to comprehend a dependable variability pattern in the germplasm assayed.

Supported by UNESCO / MIRCEN network