Molecular Biology and Genetics
  Plant Biotechnology
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 13 No. 6, Issue of November 15, 2010
© 2010 by Pontificia Universidad Católica de Valparaíso -- Chile Received May 20, 2010 / Accepted September 20, 2010
DOI: 10.2225/vol13-issue6-fulltext-13  

Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean

Flávia Fernandes Carneiro*1 · João Bosco dos Santos1 · Monik Evelin Leite1 

1Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brazil

*Corresponding author:

Financial support: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Keywords: backcross, genetic similarity, Phaseolus vulgaris, SCAR, Sclerotinia sclerotiorum, SSR assisted selection.

Abstract   Full Text

Molecular markers may accelerate selection through the identification of plants with higher proportion of recurrent parent genome, as well as identifying those plants bearing target alleles like quantitative traits loci (QTLs) for white mold resistance. The objectives of this work were: 1) to employ microsatellite markers (SSR) in order to accelerate the recovery of recurrent parent genome 2) to validate sequence characterized amplified region (SCAR) Phs associated with a QTL that confers resistance to white mold, as previously identified in bean populations. Lines G122 and M20 were crossed, which generated 267 F1 plants from backcross (BC) BC1 and 113 plants from backcross BC2.SSR polymorphic markers were adopted. The relationship between BC plants and the recurrent parent was estimated based on the recurrent genome proportion (PR) in each BC plant, and the Sorensen-Dice genetic similarity (sgir). To determine how much the phenotypic variation is explained by SCAR Phs, 56 F1:2BC1 progenies were evaluated on the field following a random block design with two replications through the straw test method. SSR markers are efficient in identifying individuals with a greater proportion of the recurrent genome. SCAR Phs was not efficient for the indirect selection of common beans for white mold resistance.

Supported by UNESCO / MIRCEN network