Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey
Semiha Erisen*1 · Mustafa Yorgancilar2 · Emine Atalay2 · Mehmet Babaoglu3 · Ahmet Duran1
1Department of Biology Education, Ahmet
Keleşoğlu Education Faculty, Selçuk University, Konya, Turkey
*Corresponding author: firstname.lastname@example.org
Financial support: This research was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) Project “Tissue Culture and Cytogenetic Studies in Some Endemic Astragalus L. Species (Leguminosae) for Germplasm Preservation and Agricultural Uses”, Number TOVAG-106O136.
Keywords: astragalus, callus, organogenesis, plant regeneration.
A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [α-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.