Fig. 1 Callus induction and shoot regeneration from leaf explants of Astragalus nezaketae. Appearance of calli on medium supplemented with:
(a) 0.2 mg/l NAA and 0.6 mg/l TDZ.
(b) 2 mg/l 2.4-D and 0.5 mg/l Kin.
(c) 0.5 mg/l NAA and 4 mg/l BA after 4 weeks of culture.
(d) Development of shoot initials on leaf explants on a medium supplemented with 0.5 mg/l NAA and 4 mg/l BA after 4 weeks of culture.
(e-f) Adventitious shoots on leaf explants on medium supplemented with 0.5 mg/l NAA and 4 mg/l BA after 8 weeks of culture.
(g) Root development on regenerated shoots after 4 weeks on rooting medium.
(h) In vitro plantlet after 10 weeks of the transfer to onto plastic pot (h), (bar = 1cm).


Supported by UNESCO / MIRCEN network