Table 1. Effects of various auxin and cytokinin combinations on callus induction frequencies of leaf and petiole explants of Astragalus nezaketae.

Growth regulators
(mg/l)

Frequency of callus
induction* (%)

Callus fresh weight
(g)

Appearance of callus

Auxin

Cytokinin

Leaf

Petiole

Mean

Leaf

Petiole

Mean

 2 2,4-D
 4 2,4-D
 8 2,4-D

 0.5 Kin
 0.5 Kin
 0.5 Kin

 100 a
 100 a
 100 a

 100 a
 100 a
 100 a

 100 a
 100 a
 100 a

 1.00
 0.97
 0.58

 1.40
 0.85
 0.79

 1.20 cd
 0.91 de
 0.68 e

Yellow Soft Smooth Callus

 0.2 NAA
 0.2 NAA
 0.2 NAA
 0.5 NAA
 0.5 NAA
 0.5 NAA

 1 BA
 2 BA
 4 BA
 1 BA
 2 BA
 4 BA

 100 a
 100 a
 100 a
 100 a
 100 a
 100 a

 100 a
 100 a
 100 a
 100 a
 100 a
 100 a

 100 a
 100 a
 100 a
 100 a
 100 a
 100 a

 0.97
 1.04
 1.14
 1.53
 1.73
 1.65

 1.71
 1.67
 1.83
 1.80
 1.77
 1.88

 1.32 bc
 1.35 bc
 1.49 ac
 1.67 ab
 1.75 a
 1.76 a

Yellowish-Green Compact Friable Calllus

 0.0 NAA
 0.0 NAA
 0.0 NAA
 0.2 NAA
 0.2 NAA
 0.2 NAA

 0.2 TDZ
 0.4 TDZ
 0.6 TDZ
 0.2 TDZ
 0.4 TDZ
 0.6 TDZ

 20 d
 40 cd
 53 bc
 100 a
 100 a
 100 a

 86 a
 26 cd
 73 ab
 100 a
 100 a
 100 a

 53 bc
 33 c
 63 b
 100 a
 100 a
 100 a

 0.03
 0.08
 0.13
 0.60
 0.66
 0.62

 0.10
 0.05
 0.16
 0.88
 0.76
 0.65

 0.07 f
 0.06 f
 0.15 f
 0.74 e
 0.71 e
 0.64 e

Yellow Friable Granular
Callus

Mean

 87

 92

 0.85 b

 1.09 a