Fig. 1 Multiple shoot induction and plant regeneration
from CN of P. Pinnata L.
(a-b)
Multiple shoot initiation from pre-conditioned CN after 2 and 3 week culture on MS
+ 1 mg l-1 of BA respectively.
(c)
Multiple shoot from pre-conditioned CN on MS + 1 mg l-1 of TDZ + 200
mg l-1 ADS.
(d)
Initiation and development of new shoots after excision of first cycle shoots
from CN (arrow).
(e-f)
Elongation of regenerated shoots on hormone free MS media with original
explant.
(g)
Rooting of regenerated shoot on MS media supplemented with 1 mg l-1 of IBA, after two weeks of culture.
(h-i)
Acclimatized plants in pot. (Bar
in (a-c) = 0.5 cm; (d-f) = 1 cm; (g-h) = 2.0 cm and I = 2.5 cm. |