Table 1. Effect of different concentrations of BA and TDZ on the frequency of shoot induction and average number of shoots from normal and preconditioned CN explants of P. pinnata (after 4 weeks of culture) on MS media.



 Plant growth
Regulators
(mg l-1)

% Shoot Induction
(Mean ± S. E.)

Mean No.
of shoots/ explant

% Shoot Induction
(Mean ± S. E.)

Mean No.
of shoots/
explant

      BA          TDZ

 Normal CN explant

Preconditioned CN explant

Control

0.0

0.0

0.0

0.0

   0.5

--

40± 1.15f

3.4 ± 0.45e

74 ± 2.14e

   7.5 ± 1.3e

1

--

59 ± 1.70b

7.3 ± 0.92b

85 ± 2.45b

12.3 ± 2.b

2

--

50 ± 1.44c

5.7 ± 1.22c

80 ± 2.31c

9.1 ± 1.6d

3

--

45 ± 1.30c

4.5 ± 0.62d

76 ± 2.19d

8.5 ± 1.3e

--

   0.5

48 ± 1.39d

4.3 ± 0.70d

80 ± 2.3c

10.5 ± 2.5c

--

1

64 ± 1.85a

8.6 ± 0.29a

90 ± 2.60a

18.4 ± 3.1a

--

2

58 ± 1.67b

6.2 ± 1.26c

85 ± 2.45b

12.6 ± 2.2b

--

3

48 ± 1.39d

5.7 ± 1.48c

78 ± 2.25d

 9.5 ± 2.3d

 

Each experiment consisted of three replicates and data were represented as Mean ± S.E. Means followed by the same letters do not differ significantly at p < 0.05 according to Duncan's multiple range test.


Supported by UNESCO / MIRCEN network