Table 2. Influence of L-glutamine and adenine sulphate on multiple shoot induction from pre-conditioned CN after 4 weeks of culture on MS media supplemented with 1 mg l-1 of TDZ.



MS media +
1 mg l-1 TDZ

Percentage
of shoot

regeneration

Mean number
of shoots/

explant

ADS (mg l-1)

L- g (mg l-1)

Control -1

64 ± 1.85c

8.6 ± 0.29e

Control -2

90 ± 2.60a

18.4 ± 3.1a

100

--

90 ± 2.60b

19.4 ± 0.31d

150

--

91 ± 2.45a

24.2 ± 0.70b

200

--

92 ± 2.66a

32.2 ± 0.93a

--

100

90 ± 2.27a

20.0 ± 0.58c

--

150

90 ± 2.52a

20.5 ± 0.52c

--

200

88 ± 0.12b

18.7 ± 0.24d

Control-1 Normal CN explant cultured on MS + 1 mg l-1 of BA.
Control-2 Preconditioned CN explant cultured on MS + 1 mg l-1 of TDZ.
Each experiment consisted of three replicates and data were represented as Mean ± S.E. Means followed by the same letters do not differ significantly at p < 0.05 according to Duncan's multiple range test.


Supported by UNESCO / MIRCEN network