Table 2. Primer pairs designed based on the flanking sequences of SSRs.


Name

GenBank accession number

Motif

Poly-
morphism

Forward primer

Tm

Reverse primer

Tm

Size

PM601

AY526357

(GGC)18

GAG GGA GCA CAA ATG AGG AA

64,1

TCT AGG TTC TCC CTG CAA CG

64,2

224

PM602

AY526358

(GA)9, (GGC)13

GCT GGG ATC TTG TGG AGC TA

64,2

AAG CCG AGG AAG GAG AAG AG

63,8

208

PM603

AY526359

(GGC)13

GGA AGG CTG CAA AAG TAA GG

62,6

CGC GCT CAT CTT AAG TCC TA

62

164

PM604

AY526360

(CCG)13

ACC ACC TCT GTG CTC TTG CT

64

GCT TCT GAT GGT GGT GGT CT

64,1

241

PM605

AY526361

(CCG)10

AGC CCT GTC AAT CAT TGG AT

63,2

CTA GTT CGG CGG CTT CAT AG

63,6

152

PM606

AY526362

(GGC)12

GCG GTG GAG GCT ACT GTA AG

63,6

CGC CGT CCT TCT CTC TAT GA

64,3

240

PM607

AY526363

(GGC)13

GGC GAC GCA ATT GAG ACT AT

64

GCC CGT GCT ACT ATC GTC AT

63,9

173

PM608

AY526364

(CCG)17

AGA ACA GGC AAA ACC CAA CA

64,3

TGG TGG TGG AAT GAG TGT TT

62,9

396

PM609

AY526365

(CCG)14

TGC CAT CTC TCT CCC TTG AA

64,8

TGG CGA CAG TGG AGA TTA GA

63,4

196

PM610

AY526366

(GGC)10

TGC TCC CCT GTT GTT ACA GA

63,3

CTG TGC TCT TGC TTA GAT CTG G

62,9

159

PM611

AY526367

(CCG)14

ATC GCA TAA CCA CCA CCA TC

64,5

GGA GGC AAC CCC ATT TTA TT

63,5

249

PM612

AY526368

(GGC)9

CAC CTC TCG GAC GTG TCA TA

63,8

TCC GTC TTC TCC TTC TCC AA

63,8

246

PM613

AY526369

(GGC)15

CTC TGG CAA CGA CGA CTG TA

64,1

TCA GAA ATG GGA CCA TGA CA

64

228

PM614

AY526370

(CCG)13

ATA GGA TGA GCT GCC CTG TT

62,5

CCG TAA GCG TAA TGG AGC AT

63,7

216

PM615

AY526371

(GGC)6

y

AGA GAG AAT CCA TGG CGA GA

63,8

ATG CTC ACC CCC TGT CTG

63,9

214

PM616

AY526372

(GGC)10

TCC ATG AAC CAG CAA ACA GA

63,8

ATT CCA ACG CAA CCA ATA CC

63,7

231

PM617

AY526373

(CCG)13

ACG ACT CCT CCA TTG TCA CC

64

GGC GAC GCA ATT GAG ACT AT

64

227

PM618

AY526374

(CCG)14, (CT)11

ATC TGC GTC AGA AGG GAG AA

63,9

GCC GAC GAG GAG ATA GTC AG

63,9

201

PM619

AY526375

(GGC)8

GGG GGT GTC TCA AAG TCT CA

64,1

CCG CGC CTA GTA TTG TCT GT

64

249

PM620

AY526376

(GGC)16

GGA GAG GGA GCA CAA ATG AA

64,1

CAC TCC AGT GTC GTT GCA CT

64,1

184

PM621

AY526377

(CCG)11

CAG AGA TCA ATG GGG AAG GA

63,9

CGG TGA AAC CTG GTG GTT AG

64,2

157

PM622

AY526378

(GGC)13

CTC CGG GAA CTC CTC TCT CT

63,8

CTC CGC AGT CAT TCA TCT CA

64,1

224

PM623

AY526379

(GGC)17

GGG CTC CAT ATG CAC TTC AG

64,5

AAC CAC AGC ATC GGA GTC TT

63,6

178

PM624

AY526380

(CCG)13

AGG GAA AGA AAC GCA GAA CA

63,6

GAA GAT GAA GGG AGC AAC CA

64,1

209

PM625

AY526381

(GGC)10

GTT GGG CTT GGT GTG ATC TT

63,9

GGT CCG CAC AGG AAA AGT TA

63,8

181

PM626

AY526382

(CCG)9

TGT GCT CTT GCT TAG ATC TGG A

63,9

GAA GCA GGG AAA GTT TGT CG

63,6

168

PM627

AY526383

(GGC)12

GGG CAG CAG AGA TAG ATT GG

63,6

TGT GCT CTT GCT TAG ATC TGG A

63,9

204

PM628

AY526384

(GGC)14

GCA AAG AAA AAC CAC GAA ACA

63,7

TCA AAG AAC TCC CTC GGC TA

63,7

207

PM629

AY526385

(CCG)15

CAA CTT CAA TCC CTG CCA TT

63,7

CCA TAG AGG TAG TGA CCA GAT ACA

60,8

250

PM630

AY526386

(CCG)12

CTC TCC GTG GCT GCT ACT TC

64

TGG CTG GTG AGA GAC AGA GA

64

152

PM631

AY526387

(GGC)10

GGT GAC GTG GTG AGA ACG AT

65,1

GTC CCT CAA TTT CAG CCA GA

64,1

228

PM632

AY526388

(CCG)6

CCA CCA AGT TGT GGT CTT GTT

63,7

AGA TCG AGG CAA GAA GCA GA

64,1

167

PM633

AY526389

(CCG)10, (CT)10

TCT AAA CCC CTG ACC TCT TCC

63,2

CGT TCG GAG GAG TGA GAG AC

64

172

PM634

AY526390

(GGC)9

GAG GGA GCA CAA ATG AGG AA

64,1

AAC CTT TGC GGA CTG ACC TA

63,5

209

PM635

AY526391

(GGC)5(GGT)2

CTG GTA GTG ATT CCG GCA GT

64

CTC CCC TAC CCT AGC CAA AC

63,5

211

PM636

AY526392

(CCG)12

AAA AGA AGG GGA AAC GAG GA

63,6

CAA ATG AAT TCA ACG CGA GA

63,6

204

PM637

AY526393

(GGC)21

TGG AAT TGC TGC TGG TAA TG

63,5

AGG ATG AAC CAT GCA CAA AA

62,9

219

PM638

AY526394

(CCG)10

ACT CGT CTG CTC ATC GTC CT

64

CCT TCT CTC TCG CGT CAA GT

63,6

213

PM639

AY526395

(CCG)15

GGA TTA ACC GCC GTC CTT AC

64,2

GTT GGC GGA GGA TGG AAG

65,7

283

PM640

AY526396

(CCG)15

CGA TGA AGA CGA AGA CGA CA

64,1

CGA GGA ACT CGA ACC AGA AG

63,8

231

PM641

AY526397

(GGC)12

GGC GAC GCA ATT GAG ACT AT

64

ACC AGA GGG CAT TGT TGT TC

63,9

195

PM642

AY526398

(CCG)15

CTC TCT TTG GCT CCA TAC GC

63,7

GGC GAC GCA ATT GAG ACT AT

64

167

PM643

AY526399

(GGC)7

GGC GAC GCA ATT GAG ACT AT

64

TCA GTT AGA GCC ACC CAT CC

64

178

PM644

AY526400

(CCG)10

CCG AGG GAT AAG AGT TTC CA

63,7

CGA CTC ACT ATA GGG CGA ATT G

63,9

202

PM645

AY526401

(CCG)11

CCT CCC CTA TGA CAC TGC TC

63,6

CTT CAA GTG AGG TGG CGA TG

65,8

155

PM646

AY526402

(GGC)13

GCA AAG CAA AAG TGG CTT CT

63,3

CCC TCT CTG CCC TAA ACA CA

64,1

220

PM647

AY526403

(GGC)11

AGC GGC TGT AAT GGA AAC AG

64

GCA ACC CAG ATT TCT TCA CC

63,4

162

PM648

AY526404

(GGC)11

ATA GCA TCG GTG ACG AGC TT

63,6

TCT CCC TCC AAC CAC CAC

63,8

215

PM649

AY526405

(GGC)17

GCG ACG CGA TTG AGA CTA TT

64,1

ACG CCT CTA TTC CGC CTA TT

63,6

158

PM650

AY526406

(CCG)9

AAC CTC TCA ATG CCC CTT CT

63,8

GTG GTG AGG GGA AGC AGA TA

64

163

PM651

AY526407

(CCG)11

CAA ACG TAA CCC ATC TGT TCT TC

66,1

CGA CTC ACT ATA GGG CGA ATT G

63

184

PM652

AY526408

(GGC)8

y

GGA AAA CCC TAA CCC CTT GA

63,7

TCT CTC TTC TCA CCC TCC TTT C

62,7

132

PM653

AY526409

(GGC)14

CTA TTC GAC GAG TTG CGA TG

63,3

CTC CCA ATC CCT CAG TTC AC

63,5

174

PM654

AY526410

(CCG)12

CGC GTT CAA GAG AGA AAA GG

63,8

GGG AGA CGA GGG AGA GTG AT

64,6

229

PM655

AY526411

(CCG)15

GCA TGA GAG CTC ACT GAA ACC

63,9

GGG TTG TTT CCT TTG CCA TA

63,5

202

PM656

AY526412

(GGC)11

GCC ACG CTC TTA AAC CTT TC

62,6

GGC GAC GCA ATT GAG ACT AT

64

238

PM657

AY526413

(CCG)11

AAC GAG GGG GAA GAA GAG AA

63,9

GGT GAT GCA GCA GAT TCA GA

64,1

216

PM658

AY526414

(GGC)11

AAT CGG AGT GCA GTG AAA CC

64

TGG CTG TGC TCT TGC TTA GA

63,8

274

PM659

AY526415

(CCG)13

ACG CAA AAA CCC TAA TCG TG

63,6

GGG GAA ACA GAA AGG AAA AA

61,7

219

PM660

AY526416

(GGC)12

CTA TTC GAC GAG TTG CGA TG

63,3

AGG TCC GCT AGG GTT TTC AT

63,5

241

PM661

AY526417

(CCG)12

y

GTT CTT CGC TGC GTC TCT CT

63,8

GCG TCT TCT CAA TCG TGT CTC

63,9

202

PM662

AY526418

(GGC)7

CGA CGC TGT TGA GAC TAT TCC

63,6

CGT GAA GGG GAT AGA TCA TTG

63,1

249

PM663

AY526419

(GGC)16

GGG AAG AGG AAG AGG AGG AA

63,5

GCA AAG GAA GGG ACA GAC AG

63,7

172

PM664

AY526420

(GGC)18

AAG CTC CAT TAA CGG TGA CG

63,7

AAA ACT CCG CCA TCA ACA TC

63,7

155

PM665

AY526421

(GGC)12

CAT GGC AAT AAC ATT CCT CCT

62,5

TTT CTG GAG CCA CCT CTG AC

64,4

206

PM666

AY526422

(GGC)10

ACA AGC TCC AGC TGA AAG GA

64

GCT TCT TCT CTC CAT CAC ACG

63,9

234

PM667

AY526423

(CCG)10

AAG CAA AAC CCT AGC CCT TC

63,2

GAG TCA TTT GCG GAG GAG AG

63,9

219

PM668

AY526424

(CCG)10

CCT ACC ACT TGC GTC CAA AT

63,5

CGA CTC ACT ATA GGG CGA ATT G

63,9

237

PM669

AY526425

(GGC)14

GCA GAC ATG AAC GAC AGT GG

64,5

ACT ACC GCC GGA AAA ACT TC

64

181

PM670

AY526426

(GGC)9

GTG CGA GAA CAG ACC TCC TC

64

AAT CAC CGT AGC GGT GTA GC

63,9

222

PM671

AY526427

(GGC)12

GAG GGA GCA CAA ATG AGG AA

64,1

CCT CAC CAA CCC TCA TTC TC

63,5

206

PM672

AY526428

(GGC)12

TGG CTA GGA AAG GTC TTC CA

63,6

TGC CAT TCT CAT CCT CTT CC

64,1

178

PM673

AY526429

(CCG)9

y

CAT CGT TTC GCA CAA AGC TA

63,8

TCT TCC ATT GAG GTT GTT GC

62,6

190

PM674

AY526430

(GGC)7, (GGC)5

TGC TCC ATT GAG ACA AGC TC

63,2

ATC CTC CGC AGC TTC CAC

65,4

206

PM675

AY526431

(CAT)24

y

AAT ACC CTT CCC CAA TCA CC

63,5

TGC TTC TGC TCG ATG TTC TG

64,3

243

PM676

AY526432

(CCG)12

GCC GGA GGA GAA ACC AAT

64

GTG CGA GAA CAG ACC TCC TC

64

216

PM677

AY526433

(GGC)10

GAC AAG GAG CAT GAC GGA AG

64,8

TCT CTC CCG ACA CAA ATT CC

64

233

PM678

AY526434

(CCG)13

TGT GCT CTT GCT TAG ATC TGG A

63,9

GAG GGA GCA CAA ATG AGG AA

64,1

221

PM679

AY526435

(GGC)13

GGC AGG GAA CCT GAA ATG TA

63,7

AGA GAA GAA GAC GCG AGA CG

63,8

179

PM680

AY526436

(GAAA)6

TGG ACT AAA AAT GGG CTT CG

63,7

TTC TCC CCG TTC TCT CAC TC

63,3

235

PM681

AY526437

(CCG)16

GGT GAG AGA GGG AGC ATC AC

63,9

CCT ATC TTT CCC GTC ACG TC

63,3

174

PM682

AY526438

(GGC)5

GAC CGA AAT CGA GAT GAA CC

63,3

TCT GCT TCT GTT TCC CAT CC

64,1

235

PM683

AY526439

(CTT)8, (CCG)10

CAC ACC GCC TTC TTC TTC TT

63,2

ATG CAG GTG TCG GAT CTT TC

64

210

PM684

AY526440

(CCG)10

AGT GCG AGA GAG GAG ACT CG

63,9

GTG GCG GAG GAG GGT AGT AG

64,7

174

PM685

AY526441

(CCG)10

ACG AGC TTC TCC CTC TCT CC

63,9

TCC GGG AAA CTC AAC AGA AG

64,1

156

PM686

AY526442

(CT)8, (CCG)10, (CT)10

CGC AAA CTT CTT TCC CTT CA

64

CGT TCG GAG GAG TGA GAG AC

64

222

PM687

AY526443

(GGC)10

TGA TAA AGG AGG CGT CTT GG

63,9

CTG TGG GGG TTC TCA CTG TC

64,7

183

PM688

AY526444

(CCG)13

GAG CTT GGT AAC GCC CAT AG

63,4

GGA GGA GGT CCA TTT GGA TT

63,9

187

PM689

AY526445

(GGC)13

TAG AAA GGC TTC CTC CAT GC

63,1

GAC ACC GCA ATC TGA CCT TT

64

172

PM690

AY526446

(GGC)10

AGA AAG AAT GGG CAG CAA AA

63,5

GAT TGG CTG GTG GAT CAC TT

63,9

204

PM691

AY526447

(CCG)11

TGC TTT TGT CCA AGG CTT CT

63,7

TGG GAC TAT TCG ACG GAG TT

63,3

216

PM692

AY526448

(CCG)13

GAA CGC GTG ATG AAG GAA AT

63,9

GTC GTC CCT CTT TGT GTC GT

64,1

233

PM693

AY526449

(GGC)9

AGC AAA GCA AAA GTG GCT TC

63,3

CCT TGA GTA TGG CTG GCT TC

63,6

174

PM694

AY526450

(GGC)14

AGT CCT GCA GGT TTA AAC GAA

62,5

TTG TTC GAC CTG TGC TCT TG

64,1

155

PM695

AY526451

(GGC)12

TGA GGG AAG AGA AGG AGA AGG

63,6

CGC TAT CCT GAG GAG GAC AC

63,7

236

PM696

AY526452

(GGC)9

AAA AGT TGA CCC ACC CAA CC

64,7

TGG TAT TGC TCC CCA CTC TC

64

197

PM697

AY526453

(CA)7, (CCG)12

CTT TCC TCA GCC TCT GAA CG

64

CGA CTA GTT GCC GAA TGA TG

63,2

243

PM698

AY526454

(GGC)6

TTT TGA GTA GGA ACC TGA GAG TTG

62,4

ACC TCT GGC GAC ACC ATC

64

151

PM699

AY526455

(GGC)14

GAG AGG GAG CAC AAA TGA GG

63,7

TCA CGA ACA TCC TCT CGT CTC

64,4

219

PM700

AY526456

(CCG)10

CAT CTG ACC TGT CAC CAT CG

64,3

GGA ACA TGA ACA GAG AGC AAC A

64,1

205

PM701

AY731521

(GGC)20

GAG GGA GCA CAA ATG AGG AA

64,1

ATC TGG ATT ACC AGC CAC CA

64,2

239

PM702

AY731522

(CA)7, (GGA)5

CTC ACC TTC GCA AAT CAC CT

64,1

ACC CCC TCT CAC TCT CCA TT

63,8

217

PM703

AY731523

(CCG)10

CAA GTC GCC GTT GCG TAG

65,6

CTT TTC CTT CTC GCG TTC TG

63,8

195

PM704

AY731524

(GGC)16

AGT CGG TCG TTG AAA ACA GG

64

AGT GCT CAG CCT TTC ATT CC

63,3

225

PM705

AY731525

(GGC)13

AGG TGA CGA ACC AAA ACG TC

63,9

TCA TTT CCA CCC CAC TAT CC

63,4

241

PM706

AY731526

(CCG)14

CCA CCC TCG TCG ATC TGT

63,9

GGA GAG GGA GCA CAA ATG AG

63,7

207

PM707

AY731527

(GGC)10, (AAG)11, (GGC)10

CTA TTC GAC GAG TTG CGA TG

63,3

GTG GTG TTG TCG CCG ATA G

64,2

217

PM708

AY731528

(GGC)6

GAG GAA GTC TCG GAG GAG GT

63,7

CTA TTC GCC TCA ATC CCT CA

63,9

224

PM710

AY731529

(CCG)9

GCA AAG CCT TGG ATC CTT TT

64,5

CTT GGC GTT TCT CTC TCT CC

64,3

219

PM711

AY731530

(CCG)9

GCA AAG CCT TGG ATC CTT TT

64,1

CTT GGC GTT TCT CTC TCT CC

63

155

PM713

AY731531

(CCG)6(ACG)2(CCG)5

GGA GAG AGA AGG GGT TGC AG

65,1

GAG AGG GAG CAC AAA TGA GG

63,7

250

PM714

AY731532

(CCG)13

TTC ATT TCC CCT CTC CTC CT

63,7

CCC CTT CTC CTG GCT TAG AT

63,3

162

PM715

AY731533

(CCG)12

CAT TGA GGC AGA GAG CTT CC

64

ATG GCA AAC GCT TTT CTC TG

64,1

212

PM716

AY731534

(CCG)7

TCT CTC CGT CCA AGA GCT TC

63,6

ACG ATG GAA CCC TCT GTC AC

64

179

PM717

AY731535

(CCG)15

AGG ATC AAG AAA AGG CAG CA

63,7

GGC GAC GCA ATT GAG ACT AT

64

164

PM718

AY731536

(CCG)13

GAA TGT CCA GCG AGA AGG AG

63,9

TGG GGA GAG AAA ACT TGC AG

64,2

161

PM719

AY731537

(GGC)11

CTT CCA CGT TCA CAC AAT GG

64,1

GGA GAG AGA GGA CGA AGA CG

63,1

222

PM721

AY731538

(GGC)10

GAG GGA GCA CAA ATG AGG AA

64,1

TAC GGT GCA TAT GCC AAA GA

63,9

243

PM722

AY731539

(GGC)13

TGG TCA AAA GGG GTA TCT GG

63,6

AAG GTC CGC TAG GAT TTT CA

62,4

168

PM723

AY731540

(GGC)11

TGG CTA GGA AAG GTC TTC CA

63,6

ATC CTC TTC CTC TGC TGC AA

64

168

PM724

AY731541

(CCG)2CC(GGC)5CG(CCG)4

ACG AGC TTC TCC CTC TTT CC

63,7

ACG CCA ATT TGG GAA CTT TT

64,2

161

PM725

AY731542

(CCG)13

AAT GAG CCC GCC ACT TTA G

64

CGT CAC TCA TCA CCA TCT CG

64,5

199

PM726

AY731543

(CCG)20

CCT TAA GCG GAA AAC CAT TG

63,1

CTC GCG GAT CTC TCT TTC TG

64,1

233

PM727

AY731544

(CCG)8

CGA GGA TCT CGA AGG GAT GT

65,4

CAA TAA CCA GCA AGC AGC AA

63,8

177

PM728

AY731545

(CCG)9

TTC CTG CAT TTC TCT CTT CCT C

63,5

AGC TCC GCA ACG AAA TAG G

64,1

173

PM730

AY731546

(CCG)10

CCA TCG TCG TCC TCA TCA T

63,7

TAG AAT CAT GCC CGC ATA CA

63,9

151

PM731

AY731547

(CCG)13

AGT CAC CGC ATC GCA CTC

65,4

CTG GGC TGG AAC TCA AAC AC

64,6

240

PM733

AY731548

(CCG)11

CCA AAT CTT CTC ATC AAG TTG C

62,4

GAG AGG GAG CAC AAA TGA GG

63,7

206

PM734

AY731549

(GGC)12

CTC GAC GCT GCT CTG TCT C

64,3

CGT TTC ATC CGA ATT CAC CT

63,7

228

PM735

AY731550

(GGC)10

y

TTA CTG GGT TAG GCG GTG AC

63,7

TCC TCT AAA GGC ACC AAA CG

63,9

154

PM736

AY731551

(GGC)14

GAG AGG GAG CAC AAA TGA GG

63,7

ACA CCG AAC AGC TTC ACT CC

64,3

212

PM737

AY731552

(GGC)10, (GGC)10

GAG CCC CCT TCT CTC TCT TC

63,3

CGT CAT CCT CAT CGT GAA TC

63,1

275

PM738

AY731553

(CCG)13

GCA CAG CAC CCT CTC TAT TTG

63,6

TGA GGG AAG AGA AGG AGA AGG

63,6

188

PM739

AY731554

(GGC)11

GAA TCA TTT GCG GAG GAG AG

63,6

CAC TAG CGG AAG TCG CTG TC

64

168

PM740

AY731555

(GGC)13

TAG AAA GGC TTC CTC CAT GC

63,1

GAC ACC GCA ATC TGA CCT TT

64

172

PM741

AY731556

(CCG)15

AGG ATC AAG AAA AGG CAG CA

63,7

GGC GAC GCA ATT GAG ACT AT

64

164

PM742

AY731557

(CCG)14

CTC AGA TCA AAG CAG CAC CA

64,2

TTC GTC CAG GGG TGG TAT TA

63,9

177

PM743

AY731558

(CCG)6

CCC AGA TCC GAG AGA ATG AG

63,7

TAA CAG GGA CGG TTT GGT TG

64,5

161

Control-1 Normal CN explant cultured on MS + 1 mg l-1 of BA.
Control-2 Preconditioned CN explant cultured on MS + 1 mg l-1 of TDZ.
Each experiment consisted of three replicates and data were represented as Mean ± S.E with significance being determined at P < 0.05 level using DMRT.

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