Fig. 2 Steps involved in constructing cDNA library and qualifying the library for different criteria.
(a) 1 µL of total RNA run on 1% agarose gel depicting the integrity of RNA represented by sharp 25S and 18S rRNA bands.
(b) Agarose gel electrophoresis of 5 µL of first strand cDNA showing the required range of cDNA sizes.
(c) 1 µL of each of first eleven collected fractions spotted on 1% agarose as compared to the spotted standard of known concentration.
(d-e) Restriction digestion analysis of 28 randomly selected clones with ApaI and EcoRV to determine percent recombination and average insert size.