|
Fig.
5 Transcriptome profiling by Real-Time PCR.
(a) Transcriptome profiling of CpEXPA1, CpEXPA2, CpEXPA3 and CpEXPA4 in C. procera roots, stem, fibers and leaves by
real time RT-PCR using equalization of unit mass method.
(b) Relative fold expression was determined by built-in software
package of BioRad iQ5 thermal cycler.
Relative quantification of transcriptome was determined by plotting the 2ΔCt values on Y-axis against different tissues of C. procera.
(c)
Lane 1-4: 1% agarose gel containing equalized concentration of RNA isolated
from root, stem, fiber and leave tissues respectively for their usage in the
subsequent cDNA synthesis and real time PCR.
(d)
Lane 1-4: PCR amplification of 18srRNA gene in roots, stem, fibers and leaves respectively
to equalize the amount of cDNA for normalization of the assay against unit
mass.
(e)
Lane 1-4: PCR amplification of β-Tubulin gene in roots, stem,
fibers and leaves respectively to equalize the amount of cDNA for normalization
of the assay against unit mass. |
