Ethanol production from sweet sorghum juice under very high gravity conditions: Batch, repeated-batch and scale up fermentation
Lakkana Laopaiboon*1,2 · Sunan Nuanpeng3 · Penjit Srinophakun4 · Preekamol Klanrit1 · Prasit Jaisil5 · Pattana Laopaiboon1
of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002
*Corresponding author: email@example.com
Financial support: Thailand Research Fund (TRF) and Commission on Higher Education, Thailand, Grant No. MRG4980199.
Keywords: batch, ethanol fermentation, repeated-batch, scale up, sweet sorghum, VHG.
Batch ethanol fermentations from sweet sorghum juice by Saccharomyces cerevisiae NP 01 were carried out in a 500 ml air-locked Erlenmeyer flask under very high gravity (VHG) and static conditions. The maximum ethanol production efficiency was obtained when 9 g l-1 of yeast extract was supplemented to the juice. The ethanol concentration (P), productivity (Qp) and yield (Yp/s) were 120.24 ± 1.35 g l-1, 3.01 ± 0.08 g l-1 h-1 and 0.49 ± 0.01, respectively. Scale up ethanol fermentation in a 5-litre bioreactor at an agitation rate of 100 rev min-1 revealed that P, Qp and Yp/s were 139.51 ± 0.11 g l-1, 3.49 ± 0.00 g l-1 h-1 and 0.49 ± 0.01, respectively, whereas lower P (119.53 ± 0.20 g l-1) and Qp (2.13 ± 0.01 g l-1 h-1) were obtained in a 50-litre bioreactor. In the repeated-batch fermentation in the 5-litre bioreactor with fill and drain volume of 50% of the working volume, lower P and Qp were observed in the subsequent batches. P in batch 2 to 8 ranged from 103.37 ± 0.28 to 109.53 ± 1.06 g l-1.