Fig. 1 (a) Chromatogram showing the fatty acid composition of three different ol genotypes (Ol₁Ol₁Ol₂Ol₂, Ol₁ol₁Ol₂ol₂, and ol₁ol₁ol₂ol₂,). The x-axis represents the retention time. Eight fatty acids were revealed for each sample; however, palmitic (C16:0), oleic (C18:1), and linoleic (C18:2) were the predominant fatty acids detected in these samples.
(b) Amplification plots of the three FAD2A genotypes (Ol₁Ol₁, ol₁ol₁, and Ol₁ol₁) detected with real-time PCR using two TaqMan^® probes to target the wild type and mutant allele (G448A) in FAD2A. The first amplification plot shows the homozygous dominant genotype, the middle plot is homozygous recessive, and the bottom plot is heterozygous. The x-axis represents the cycle number; whereas, the y-axis represents the normalized fluorescence.