Fig. 2 Detection of TSWV L gene by RT-PCR with primers L1 TSWV/L2 TSWV.
(a) RT-PCR products showing 276 bp amplicon in naturally infected I. hawkerii.
(b) After elimination of virus. Lane M, MassRuler DNA ladder, Low Range (Fermentas Life Sciences GmbH, Lithuania); lanes 1 to 4, samples; lane 5, positive control; lane 6, negative control, Rnase free water insteаd target RNA; lane 7 - negative control, healthy I. hawkerii plants.


Supported by UNESCO / MIRCEN network