Table 1 - Allelic variation at the Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3 and Ppd-D1a loci of Pakistani spring wheat cultivars

Table 1. Primer sequences, annealing temperatures and expected PCR product sizes for detecting alleles at major Vrn and Ppd-D1 loci in wheat. (No citada en texto)

Locus	Allele	Primers	Primer sequence (5′─3′)	Expected PCR product size (bp)	Annealing Temperature (°C)
	Vrn-A1a Vrn-A1b vrn-A1	VRN1AF VRN1R	GAAAGGAAAAATTCTGCTCG TGCACCTTCCC(C/G)CGCCCCAT	650+750 ≈480 ≈500	55
Vrn-A1	Vrn-A1c	Intr1/A/F2 Intr1/A/R3	AGCCTCCACGGTTTGAAAGTAA AAGTAAGACAACACGAATGTGAGA	1170	57
	vrn-A1	Intr1/C/F Intr1/AB/R	GCACTCCTAACCCACTAACC TCATCCATCATCAAGGCAAA	1068	57
Vrn-B1	Vrn-B1	Intr1/B/F Intr1/B/R3	CAAGTGGAACGGTTAGGACA CTCATGCCAAAAATTGAAGATGA	709	58
Vrn-B1	vrn-B1	Intr1/B/F Intr1/B/R4	CAAGTGGAACGGTTAGGACA CAAATGAAAAGGAATGAGAGCA	1149	58
Vrn-D1	Vrn-D1	Intr1/D/F Intr1/D/R3	GTTGTCTGCCTCATCAAATCC GGTCACTGGTGGTCTGTGC	1671	61
Vrn-D1	vrn-D1	Intr1/D/F Intr1/D/R4	GTTGTCTGCCTCATCAAATCC AAATGAAAAGGAACGGAGCG	997	56
Vrn-B3	Vrn-B3	VRN4-B-INS-F VRN4-B-INS-R	CATAATGCCAAGCCGGTGAGTAC ATGTCTGCCAATTAGCTAGC	≈1200	63
Vrn-B3	vrn-B3	VRN4-B-NOINS-F VRN4-B-NOINS-R	ATGCTTTCGCTTGCCATCC CTATCCCTACCGGCCATTAG	≈1140	57
	Ppd-D1a	Ppd-D1_F	ACGCCTCCCACTACACTG	414
Ppd-D1		Ppd-D1_R1	TTGGTTCAAACAGAGAGC		54
	ppd-D1a	Ppd-D1_R2	CACTGGTGGTAGCTGAGATT	288

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