Table 2 - Selection of reference genes for normalization of quantitative real-time PCR in cell cultures of Cyclamen persicum

Table 2. Primer sequences, primer concentrations, product sizes and annealing temperatures.

Abbreviated Gene Designation	Putative Gene Product	Genbank	Forward Primer (F) Reverse Primer (R) 5’ → 3’	Product Size (Bp)	Annealing Temp (ºC)	Primer Concentration (Nm)
Ef-Tu	Elongation factor tu	AJ886626	F: TATCCAGAGGGGGATGGTT R: TGCCTACCTCCCTCTTCCT	102	59	200
ABC transporter ATPase	ABC transporter ATPase	AJ886326	F:TGGCGAAACGTATTGAGAA R:AGTTGCTGGGGTTAGCATTT	98	59	100
V-ATPase G subunit 1	V-ATPase G subunit 1	AJ887447	F: AGATCGGGTGCTAATGTGA R: AATCCCAGCATCGTTTTTCT	79	59	400
COG complex component	putative conserved oligomeric Golgi (COG) complex component	AJ887962	F:TATCCAACGCCGACAAAATA R:GGAATGCTTCGATTTTTGCT	105	59	200
H3-K9-HMTase 4	Histone H3-K9 methyltransferase 4	AJ885940	F: GGTGTGAGATCTTGGGATT R: GTCAAGCCCCTTCATTGTTT	148	59	200
PRK	putative receptor kinase	AJ886123	F: GTGGTGAGAGAAGAATGGA R: GCATTTTAGGCCTCTTTTCG	107	59	400

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